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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
24/05/2022 |
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Actualizado : |
24/05/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RAFFO, M.A.; AZZIMONTI, G.; PEREYRA, S.; PRITSCH, C.; LADO, B.; DREISIGACKER, S.; QUINCKE, M.; CASTRO, A.; SILVA, P.; GARCIA, R.; PEREIRA, F.; GERMAN, S. |
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Afiliación : |
MIGUEL ANGEL RAFFO BUSCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO AZZIMONTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLARA PRITSCH, Facultad de Agronomía, Universidad de la República, Garzón 780, CP 12900 Montevideo, Uruguay.; BETTINA LADO, Facultad de Agronomía, Universidad de la República, Garzón 780, CP 12900 Montevideo, Uruguay.; International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600 Mexico, DF, Mexico.; MARTIN CONRADO QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Facultad de Agronomía, Universidad de la República, Garzón 780, CP 12900 Montevideo, Uruguay.; MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICHARD ANSELMO GARCIA USUCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO PEREIRA CALISTRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Introgression of the coupled Fhb1-Sr2 to increase Fusarium head blight and stem rust resistance of elite wheat cultivars. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Plant Genetic Resources: Characterization and Utilization, 1-10, 2022. Doi: https://doi.org/10.1017/S1479262122000107 |
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DOI : |
10.1017/S1479262122000107 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 1 June 2021/Revised: 20 April 2022/Accepted: 20 April 2022. Author for correspondence: S. Germán, E-mail: sgerman@inia.org.uy. The supplementary material for this article can be found at https://doi.org/10.1017/S1479262122000107 |
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Contenido : |
Abstract:
Fusarium head blight (FHB) and stem rust (SR) threaten the sustainability of wheat production worldwide. Fhb1 and Sr2 confer partial durable resistance to FHB and SR, respectively. Despite resistant alleles of both genes are linked in repulsion, lines with Fhb1-Sr2 in coupling were developed at the University of Minnesota, USA. Marker-assisted backcrossing was used to incorporate the coupled Fhb1-Sr2 into four elite INIA-Uruguay spring wheat varieties lacking both genes and expressing different levels of FHB and SR resistance. In each case, the initial cross between the donor line and recurrent parent was backcrossed three times. Genotypes carrying Fhb1-Sr2 were selected using the molecular marker UMN10. In BC3F3 families, retention of Fhb1-Sr2 was further confirmed with the markers SNP3BS-8 and Sr2-ger9 for Fhb1 and Sr2, respectively. BC3F3 homozygous lines contrasting at UMN10, SNP3BS-8 and Sr2-ger9 were obtained to quantify the effect of Fhb1-Sr2 on the resistance to FHB under controlled conditions and to SR under field conditions. After 26 months period, successful introgression of Fhb1-Sr2 into the four cultivars was achieved, representing novel wheat genetic resources. Lines homozygous for the resistant alleles of Fhb1 were significantly more resistant to FHB as reflected by an 18% reduction of average FHB area under the disease progress curve. A significant effect of Sr2 on SR field resistance was observed in lines derived from the most susceptible cultivar ?Génesis 2375?. The most resistant lines to both diseases are expected to be valuable genetic resources in breeding for durable resistance to FHB and SR. MenosAbstract:
Fusarium head blight (FHB) and stem rust (SR) threaten the sustainability of wheat production worldwide. Fhb1 and Sr2 confer partial durable resistance to FHB and SR, respectively. Despite resistant alleles of both genes are linked in repulsion, lines with Fhb1-Sr2 in coupling were developed at the University of Minnesota, USA. Marker-assisted backcrossing was used to incorporate the coupled Fhb1-Sr2 into four elite INIA-Uruguay spring wheat varieties lacking both genes and expressing different levels of FHB and SR resistance. In each case, the initial cross between the donor line and recurrent parent was backcrossed three times. Genotypes carrying Fhb1-Sr2 were selected using the molecular marker UMN10. In BC3F3 families, retention of Fhb1-Sr2 was further confirmed with the markers SNP3BS-8 and Sr2-ger9 for Fhb1 and Sr2, respectively. BC3F3 homozygous lines contrasting at UMN10, SNP3BS-8 and Sr2-ger9 were obtained to quantify the effect of Fhb1-Sr2 on the resistance to FHB under controlled conditions and to SR under field conditions. After 26 months period, successful introgression of Fhb1-Sr2 into the four cultivars was achieved, representing novel wheat genetic resources. Lines homozygous for the resistant alleles of Fhb1 were significantly more resistant to FHB as reflected by an 18% reduction of average FHB area under the disease progress curve. A significant effect of Sr2 on SR field resistance was observed in lines derived from the most susceptible cultivar ... Presentar Todo |
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Palabras claves : |
FUSARIUM HEAD BLIGHT; STEM RUST; TRITICUM AESTIVUM; UMN10. |
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Thesagro : |
TRIGO. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02904naa a2200337 a 4500
001 1063162
005 2022-05-24
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1017/S1479262122000107$2DOI
100 1 $aRAFFO, M.A.
245 $aIntrogression of the coupled Fhb1-Sr2 to increase Fusarium head blight and stem rust resistance of elite wheat cultivars.$h[electronic resource]
260 $c2022
500 $aArticle history: Received: 1 June 2021/Revised: 20 April 2022/Accepted: 20 April 2022. Author for correspondence: S. Germán, E-mail: sgerman@inia.org.uy. The supplementary material for this article can be found at https://doi.org/10.1017/S1479262122000107
520 $aAbstract: Fusarium head blight (FHB) and stem rust (SR) threaten the sustainability of wheat production worldwide. Fhb1 and Sr2 confer partial durable resistance to FHB and SR, respectively. Despite resistant alleles of both genes are linked in repulsion, lines with Fhb1-Sr2 in coupling were developed at the University of Minnesota, USA. Marker-assisted backcrossing was used to incorporate the coupled Fhb1-Sr2 into four elite INIA-Uruguay spring wheat varieties lacking both genes and expressing different levels of FHB and SR resistance. In each case, the initial cross between the donor line and recurrent parent was backcrossed three times. Genotypes carrying Fhb1-Sr2 were selected using the molecular marker UMN10. In BC3F3 families, retention of Fhb1-Sr2 was further confirmed with the markers SNP3BS-8 and Sr2-ger9 for Fhb1 and Sr2, respectively. BC3F3 homozygous lines contrasting at UMN10, SNP3BS-8 and Sr2-ger9 were obtained to quantify the effect of Fhb1-Sr2 on the resistance to FHB under controlled conditions and to SR under field conditions. After 26 months period, successful introgression of Fhb1-Sr2 into the four cultivars was achieved, representing novel wheat genetic resources. Lines homozygous for the resistant alleles of Fhb1 were significantly more resistant to FHB as reflected by an 18% reduction of average FHB area under the disease progress curve. A significant effect of Sr2 on SR field resistance was observed in lines derived from the most susceptible cultivar ?Génesis 2375?. The most resistant lines to both diseases are expected to be valuable genetic resources in breeding for durable resistance to FHB and SR.
650 $aTRIGO
653 $aFUSARIUM HEAD BLIGHT
653 $aSTEM RUST
653 $aTRITICUM AESTIVUM
653 $aUMN10
700 1 $aAZZIMONTI, G.
700 1 $aPEREYRA, S.
700 1 $aPRITSCH, C.
700 1 $aLADO, B.
700 1 $aDREISIGACKER, S.
700 1 $aQUINCKE, M.
700 1 $aCASTRO, A.
700 1 $aSILVA, P.
700 1 $aGARCIA, R.
700 1 $aPEREIRA, F.
700 1 $aGERMAN, S.
773 $tPlant Genetic Resources: Characterization and Utilization, 1-10, 2022. Doi: https://doi.org/10.1017/S1479262122000107
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
19/04/2023 |
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Actualizado : |
14/09/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CABRERA, A.; PITA, S.; GONZÁLEZ, T.; VIERA, A.; VERGER, L.; PIEGAS, S.; WILLAT, G.; FRESIA, P.; BASMADJIÁN, Y. |
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Afiliación : |
ANDRÉS CABRERA, Dpto. Parasitología y Micología, Inst.Higiene, Fac. Medicina, UdelaR, Mdeo, Uruguay; Lab. Interacciones Hospedero-Patógeno, Inst.Pasteur Mdeo, Mdeo, Uruguay; Unid. Microbiología, Dpto. Patobiología, Fac. Veterinaria, UdelaR, Mdeo, Uruguay.; SEBASTIÁN PITA, Laboratorio de Interacciones Hospedero-Patógeno, Institut Pasteur Montevideo, Montevideo, Uruguay Sección Genética Evolutiva, Facultad de Ciencias, UdelaR, Montevideo, Uruguay; TELMA GONZÁLEZ, Departamento de Parasitología y Micología, Instituto de Higiene, Facultad de Medicina, UdelaR, Montevideo, Uruguay; ANA VIERA, Departamento de Parasitología y Micología, Instituto de Higiene, Facultad de Medicina, UdelaR, Montevideo, Uruguay; LORENZO VERGER, Ministerio de Salud Pública, Uruguay, Montevideo, Uruguay; SOFÍA PIEGAS, Ministerio de Salud Pública, Uruguay, Montevideo, Uruguay; GABRIELA WILLAT, Ministerio de Salud Pública, Uruguay, Montevideo, Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; YESTER BASMADJIÁN, Departamento de Parasitología y Micología, Instituto de Higiene, Facultad de Medicina, UdelaR, Montevideo, Uruguay. |
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Título : |
Genetic variability highlights the invasion route of the Lutzomyia longipalpis complex, the main vector of Visceral Leishmaniasis in Uruguay. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Zoonoses and Public Health. 2023, Volume 70, Issue 5, Pages 383-392. doi: https://doi.org/10.1111/zph.13036 |
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ISSN : |
1863-1959 |
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DOI : |
10.1111/zph.13036 |
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Idioma : |
Inglés |
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Notas : |
Article history: First published 10 March 2023. -- Correspondence author: Andrés, C.; Departamento de Parasitología y Micología, Instituto de Higiene, Facultad de Medicina, UdelaR, Montevideo, Uruguay; email:andresmcc1@gmail.com -- Publisher: John Wiley and Sons Inc. -- Supplementary material available. -- |
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Contenido : |
In the Americas, the sandfly Lutzomyia longipalpis is the main vector of the parasitic protozoa Leishmania infantum, the etiological agent of visceral leishmaniasis (VL). The Lu. longipalpis species complex is currently discontinuously distributed across the Neotropical region, from Mexico to the north of Argentina and Uruguay. During its continental spreading, it must have adapted to several biomes and temperature amplitudes, when founder events should have contributed to the high genetic divergence and geographical structure currently observed, reinforcing the speciation process. The first report of Lu. longipalpis in Uruguay was in 2010, calling the attention of Public Health authorities. Five years later, the parasite Le. infantum was recorded and in 2015 the first case of VL in canids was reported. Hitherto seven human deaths by VL have been reported in Uruguay. Here, we publish the first DNA sequences from the mitochondrial genes ND4 and CYTB of Lu. longipalpis collected in Uruguay, and we used these molecular markers to investigate their genetic variability and population structure. We described four new ND4 haplotypes in a total of 98 (4/98) and one CYTB in a total of 77 (1/77). As expected, we were able to establish that the Lu. longipalpis collected in two localities (i.e. Salto and Bella Unión) from the north of Uruguay are closely related to the populations from neighbouring countries. We also propose that the possible route for the vector arrival to the region may have been through vegetation and forest corridors of the Uruguay River system, as well as it may have benefited from landscape modifications generated by commercial forestation. The ecological-scale processes shaping Lu. longipalpis populations, the identification of genetically homogeneous groups and the gene flow among them must be carefully investigated by using highly sensible molecular markers (i.e. genome wide SNPs) since it will help to the understanding of VL transmission and contribute to the planification of public policies on its control. © 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd. MenosIn the Americas, the sandfly Lutzomyia longipalpis is the main vector of the parasitic protozoa Leishmania infantum, the etiological agent of visceral leishmaniasis (VL). The Lu. longipalpis species complex is currently discontinuously distributed across the Neotropical region, from Mexico to the north of Argentina and Uruguay. During its continental spreading, it must have adapted to several biomes and temperature amplitudes, when founder events should have contributed to the high genetic divergence and geographical structure currently observed, reinforcing the speciation process. The first report of Lu. longipalpis in Uruguay was in 2010, calling the attention of Public Health authorities. Five years later, the parasite Le. infantum was recorded and in 2015 the first case of VL in canids was reported. Hitherto seven human deaths by VL have been reported in Uruguay. Here, we publish the first DNA sequences from the mitochondrial genes ND4 and CYTB of Lu. longipalpis collected in Uruguay, and we used these molecular markers to investigate their genetic variability and population structure. We described four new ND4 haplotypes in a total of 98 (4/98) and one CYTB in a total of 77 (1/77). As expected, we were able to establish that the Lu. longipalpis collected in two localities (i.e. Salto and Bella Unión) from the north of Uruguay are closely related to the populations from neighbouring countries. We also propose that the possible route for the vector arrival to the region m... Presentar Todo |
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Palabras claves : |
Infectious disease; Life on land (SDS Goal 15); Lutzomyia longipalpis complex; Molecular markers; MtDNA; Phylogeography; UNIDAD MIXTA PASTEUR + INIA. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 03480naa a2200337 a 4500
001 1064042
005 2023-09-14
008 2023 bl uuuu u00u1 u #d
022 $a1863-1959
024 7 $a10.1111/zph.13036$2DOI
100 1 $aCABRERA, A.
245 $aGenetic variability highlights the invasion route of the Lutzomyia longipalpis complex, the main vector of Visceral Leishmaniasis in Uruguay.$h[electronic resource]
260 $c2023
500 $aArticle history: First published 10 March 2023. -- Correspondence author: Andrés, C.; Departamento de Parasitología y Micología, Instituto de Higiene, Facultad de Medicina, UdelaR, Montevideo, Uruguay; email:andresmcc1@gmail.com -- Publisher: John Wiley and Sons Inc. -- Supplementary material available. --
520 $aIn the Americas, the sandfly Lutzomyia longipalpis is the main vector of the parasitic protozoa Leishmania infantum, the etiological agent of visceral leishmaniasis (VL). The Lu. longipalpis species complex is currently discontinuously distributed across the Neotropical region, from Mexico to the north of Argentina and Uruguay. During its continental spreading, it must have adapted to several biomes and temperature amplitudes, when founder events should have contributed to the high genetic divergence and geographical structure currently observed, reinforcing the speciation process. The first report of Lu. longipalpis in Uruguay was in 2010, calling the attention of Public Health authorities. Five years later, the parasite Le. infantum was recorded and in 2015 the first case of VL in canids was reported. Hitherto seven human deaths by VL have been reported in Uruguay. Here, we publish the first DNA sequences from the mitochondrial genes ND4 and CYTB of Lu. longipalpis collected in Uruguay, and we used these molecular markers to investigate their genetic variability and population structure. We described four new ND4 haplotypes in a total of 98 (4/98) and one CYTB in a total of 77 (1/77). As expected, we were able to establish that the Lu. longipalpis collected in two localities (i.e. Salto and Bella Unión) from the north of Uruguay are closely related to the populations from neighbouring countries. We also propose that the possible route for the vector arrival to the region may have been through vegetation and forest corridors of the Uruguay River system, as well as it may have benefited from landscape modifications generated by commercial forestation. The ecological-scale processes shaping Lu. longipalpis populations, the identification of genetically homogeneous groups and the gene flow among them must be carefully investigated by using highly sensible molecular markers (i.e. genome wide SNPs) since it will help to the understanding of VL transmission and contribute to the planification of public policies on its control. © 2023 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.
653 $aInfectious disease
653 $aLife on land (SDS Goal 15)
653 $aLutzomyia longipalpis complex
653 $aMolecular markers
653 $aMtDNA
653 $aPhylogeography
653 $aUNIDAD MIXTA PASTEUR + INIA
700 1 $aPITA, S.
700 1 $aGONZÁLEZ, T.
700 1 $aVIERA, A.
700 1 $aVERGER, L.
700 1 $aPIEGAS, S.
700 1 $aWILLAT, G.
700 1 $aFRESIA, P.
700 1 $aBASMADJIÁN, Y.
773 $tZoonoses and Public Health. 2023, Volume 70, Issue 5, Pages 383-392. doi: https://doi.org/10.1111/zph.13036
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